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J Microbiol Methods. 2014 Dec;107:66-70. doi: 10.1016/j.mimet.2014.09.003. Epub 2014 Sep 16.

Development of an appropriate PCR system for the reclassification of Streptococcus suis.

Author information

1
Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
2
Department of Biotechnology, Nong Lam University, Quarter 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam.
3
Department of Bioresource Sciences, Graduate School of Agricultural Sciences, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe, Hyogo 657-8501, Japan.
4
Organization for Advanced Science and Technology, Kobe University, Rokko-dai 1-1, Nada-ku, Kobe, Hyogo 657-8501, Japan.
5
Department of Microbiology, Aichi Gakuin University, School of Pharmacy, 1-100 Kusumoto-cho, Nagoya, Aichi 464-8650, Japan.
6
Department of Epizootiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.
7
Department of Infection Control Science and Department of Bacteriology, Faculty of Medicine, Juntendo University, Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
8
Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address: asekizak@mail.ecc.u-tokyo.ac.jp.

Abstract

Thirty-five serotypes of Streptococcus suis (serotypes 1-34 and serotype 1/2) have so far been described on the basis of their polysaccharide capsular antigens. However, in the last decade, some serotype reference strains have been reexamined for their taxonomic status, and the reference strains of serotypes 20, 22, 26, 32, 33, and 34 may be different from taxon S. suis. In the present study, we developed a novel PCR method targeting the recombination/repair protein (recN) gene of S. suis, designated recN PCR, which corresponds to the current reclassification of this bacterium. We compared its specificity with other PCR methods for S. suis, and the results obtained confirmed its specificity. In addition, the detection limits of recN PCR were similar among all the reference strains of authentic S. suis, indicating that the recN PCR gave reliable results against bacterial strains and isolates used in this study. Therefore, recN PCR described in the present study will be a useful tool for the identification of authentic S. suis, and can also be used in epidemiological studies on this bacterium.

KEYWORDS:

Diagnosis; PCR assay; Reclassification; Streptococcus suis; recN gene

PMID:
25229648
DOI:
10.1016/j.mimet.2014.09.003
[Indexed for MEDLINE]

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