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Indian Pediatr. 2014 Sep;51(9):727-9.

Molecular copro-prevalence of Cryptosporidium in Egyptian children and evaluation of three diagnostic methods.

Author information

1
Departments of Clinical and Chemical Pathology, and *Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Cairo, Egypt. Correspondence to: Dr Ayman A El-Badry, Medical Parasitology Department,  Kasr Al-Ainy Faculty of Medicine, Cairo University, El Manial, Cairo, Egypt. aelbadry@kasralainy.edu.eg.

Abstract

OBJECTIVE:

To determine molecular prevalence of Cryptosporidium in a cohort of Egyptian children and compare three diagnostic tests.

METHODS:

Stool samples from children with diarrhea (n=150) and from apparently healthy children (n=100) were examined for Cryptosporidium using microscopy, enzyme linked immuosorbant assay (ELISA) and nested polymerase chain reaction (nPCR).

RESULTS:

nPCR detected Cryptosporidium in 22.4% of children. Acid-fast stain and ELISA showed false negativity but 100% specificity with nPCR as gold standard.

CONCLUSION:

Cryptosporidium is a common cause of diarrhea in children in Egypt.

PMID:
25228606
DOI:
10.1007/s13312-014-0490-0
[Indexed for MEDLINE]
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