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Magn Reson Med. 2015 Sep;74(3):772-84. doi: 10.1002/mrm.25435. Epub 2014 Sep 16.

Assessing tumor cytoarchitecture using multiecho DSC-MRI derived measures of the transverse relaxivity at tracer equilibrium (TRATE).

Semmineh NB1,2, Xu J1,3, Skinner JT1,3, Xie J1, Li H1,2, Ayers G4, Quarles CC1,2,3,5,6.

Author information

1
Institute of Imaging Science, Vanderbilt University, Nashville, Tennessee, USA.
2
Department of Physics and Astronomy, Vanderbilt University, Nashville, Tennessee, USA.
3
Department of Radiology and Radiological Sciences, Vanderbilt University, Nashville, Tennessee, USA.
4
Department of Biostatistics, Vanderbilt University, Nashville, Tennessee, USA.
5
Department of Biomedical Engineering, Vanderbilt University, Nashville, Tennessee, USA.
6
Department of Cancer Biology, Vanderbilt University, Nashville, Tennessee, USA.

Abstract

PURPOSE:

In brain tumor dynamic susceptibility contrast (DSC)-MRI studies, multiecho acquisition methods are used to quantify the dynamic changes in T1 and T2 * that occur when contrast agent (CA) extravasates. Such methods also enable the estimation of the effective tissue CA transverse relaxivity. The goal of this study was to evaluate the sensitivity of the transverse relaxivity at tracer equilibrium (TRATE) to tumor cytoarchitecture.

METHODS:

Computational and in vitro studies were used to evaluate the biophysical basis of TRATE. In 9L, C6, and human brain tumors, TRATE, the apparent diffusion coefficient (ADC), the CA transfer constant (K(trans) ), the extravascular extracellular volume fraction (ve ), and histological data were compared.

RESULTS:

Simulations and in vitro results indicate that TRATE is highly sensitive to variations in cellular properties such as cell size and density. The histologic cell density and TRATE values were significantly higher in 9L tumors as compared to C6 tumors. In animal and human tumors, a voxel-wise comparison of TRATE with ADC, ve , and K(trans) maps showed low spatial correlation.

CONCLUSION:

The assessment of TRATE is clinically feasible and its sensitivity to tissue cytoarchitectural features not present in other imaging methods indicate that it could potentially serve as a unique structural signature or "trait" of cancer.

KEYWORDS:

cellular structures; contrast agent equilibrium; contrast agent leakage; dynamic susceptibility contrast; transverse relaxation; vascular structures

PMID:
25227668
PMCID:
PMC4362846
DOI:
10.1002/mrm.25435
[Indexed for MEDLINE]
Free PMC Article

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