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Urol Int. 2015;94(4):479-84. doi: 10.1159/000365197. Epub 2014 Sep 6.

Identification of frequent differentially methylated region in sporadic bladder cancers.

Author information

1
Division of Urology, Nihon University School of Medicine, Tokyo, Japan.

Abstract

INTRODUCTION:

Aberrant methylation levels in the cytosine-phosphate-guanine island (CpGi) region from exon 1 to intron 1 of the zygote arrest 1 (ZAR1) gene have been reported in several types of human cancers, including melanoma, brain tumor, and hepatocellular carcinoma. In the present study, methylation levels at the CpGi of ZAR1 exon 1/intron 1 in bladder cancer specimens were analyzed using mass spectrometry.

MATERIALS AND METHODS:

Genomic DNA was extracted from 20 sporadic bladder cancers, and the methylation levels at ZAR1 CpGi were quantitatively examined by the MassARRAY EpiTYPER method.

RESULT:

The methylation levels at specific CpG sites of the ZAR1 CpGi were significantly lower in high-grade bladder cancers than in low-grade tumors.

CONCLUSIONS:

The results of the present study indicated a decreased methylation level at CpG sites of ZAR1 exon 1/intron 1. CpGi could serve as a biomarker for invasive bladder cancer.

PMID:
25227574
DOI:
10.1159/000365197
[Indexed for MEDLINE]

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