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J Vis Exp. 2014 Sep 8;(91):e51675. doi: 10.3791/51675.

Design and fabrication of ultralight weight, adjustable multi-electrode probes for electrophysiological recordings in mice.

Author information

1
The Neuroscience Institute, New York University Langone Medical Center.
2
Department of Brain and Cognitive Science, Massachusetts Institute of Technology.
3
The Neuroscience Institute, New York University Langone Medical Center; michael.halassa@nyumc.org.

Abstract

The number of physiological investigations in the mouse, mus musculus, has experienced a recent surge, paralleling the growth in methods of genetic targeting for microcircuit dissection and disease modeling. The introduction of optogenetics, for example, has allowed for bidirectional manipulation of genetically-identified neurons, at an unprecedented temporal resolution. To capitalize on these tools and gain insight into dynamic interactions among brain microcircuits, it is essential that one has the ability to record from ensembles of neurons deep within the brain of this small rodent, in both head-fixed and freely behaving preparations. To record from deep structures and distinct cell layers requires a preparation that allows precise advancement of electrodes towards desired brain regions. To record neural ensembles, it is necessary that each electrode be independently movable, allowing the experimenter to resolve individual cells while leaving neighboring electrodes undisturbed. To do both in a freely behaving mouse requires an electrode drive that is lightweight, resilient, and highly customizable for targeting specific brain structures. A technique for designing and fabricating miniature, ultralight weight, microdrive electrode arrays that are individually customizable and easily assembled from commercially available parts is presented. These devices are easily scalable and can be customized to the structure being targeted; it has been used successfully to record from thalamic and cortical regions in a freely behaving animal during natural behavior.

PMID:
25225749
PMCID:
PMC4309135
DOI:
10.3791/51675
[Indexed for MEDLINE]
Free PMC Article

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