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Cryobiology. 2014 Dec;69(3):496-9. doi: 10.1016/j.cryobiol.2014.09.001. Epub 2014 Sep 16.

Cytochalasin B efficiency in the cryopreservation of immature bovine oocytes by Cryotop and solid surface vitrification methods.

Author information

1
Embryo Technology and Stem Cell Research Center, Thailand; School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
2
Embryo Technology and Stem Cell Research Center, Thailand; School of Anatomy, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
3
Embryo Technology and Stem Cell Research Center, Thailand; School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand. Electronic address: rangsun@g.sut.ac.th.

Abstract

The present study was undertaken to compare the efficacies of Cryotop (CT), solid surface vitrification (SSV) methods and cytochalasin B (CB) treatment for the cryopreservation of immature bovine oocytes, in terms of survival, nuclear maturation, and in vitro development. Solution exposed oocytes were in vitro maturated and fertilized. No difference was found in the rates of survival, nuclear maturation and blastocyst among solution exposed groups and fresh control group, except blastocysts rates in oocytes exposed to CB, cryoprotectant (CPA) and fluorescein diacetate (FDA) group (CB-CPA-FDA) (23%) significantly lower than that of control group (32%). CB pretreated ((+)CB) or non-pretreated ((-)CB) COCs were vitrified either by SSV or CT. Among four vitrified groups the nuclear maturation rates (CT(-)CB: 58%, CT(+)CB: 57%, SSV(-)CB: 60%, SSV(+)CB: 63%), cleavage (CT(-)CB: 36%, CT(+)CB: 24%, SSV(-)CB: 34%, SSV(+)CB: 26%) and blastocysts rates (CT(-)CB: 6%, CT(+)CB: 7%, SSV(-)CB: 4%, SSV(+)CB: 6%) did not differ, but the rates of the four vitrified groups were significantly lower than those of non-vitrified group (81%, 71% and 26%, respectively). We thus conclude that CT and SSV perform equally in vitrification of bovine immature oocytes, and CB did not increase the viability, nuclear maturation, or in vitro development of vitrified oocytes.

KEYWORDS:

Bovine; Cryotop; Immature oocytes; Solid surface vitrification; Vitrification

PMID:
25224047
DOI:
10.1016/j.cryobiol.2014.09.001
[Indexed for MEDLINE]

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