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Antimicrob Agents Chemother. 2014 Dec;58(12):7538-40. doi: 10.1128/AAC.03870-14. Epub 2014 Sep 15.

Evaluation of a loop-mediated isothermal amplification-based methodology to detect carbapenemase carriage in Acinetobacter clinical isolates.

Author information

1
Department of Clinical Microbiology, CDB, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona, Spain.
2
Centre for International Health Research (CRESIB-Hospital Clinic), Barcelona, Spain.
3
Servicio de Microbiología, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.
4
Department of Clinical Microbiology, CDB, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona, Spain Centre for International Health Research (CRESIB-Hospital Clinic), Barcelona, Spain.
5
Department of Clinical Microbiology, CDB, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona, Spain Centre for International Health Research (CRESIB-Hospital Clinic), Barcelona, Spain jvila@ub.edu.

Abstract

Carbapenem-resistant Acinetobacter baumannii is a major source of nosocomial infections worldwide and is mainly associated with the acquisition of OXA-type carbapenemases and, to a lesser extent, metallo-β-lactamases (MBLs). In this study, 82 nonepidemiologically related Acinetobacter strains carrying different types of OXA or MBL enzymes were tested using the Eazyplex system, a loop-mediated isothermal amplification (LAMP)-based method to rapidly detect carbapenemase carriage. The presence/absence of carbapenem-hydrolyzing enzymes was correctly determined for all isolates in <30 min.

PMID:
25224010
PMCID:
PMC4249531
DOI:
10.1128/AAC.03870-14
[Indexed for MEDLINE]
Free PMC Article

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