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Neural Regen Res. 2014 Jul 15;9(14):1351-7. doi: 10.4103/1673-5374.137587.

An inside-out vein graft filled with platelet-rich plasma for repair of a short sciatic nerve defect in rats.

Author information

1
Department of Orthopedic Surgery, Seoul Paik Hospital, Inje University, Seoul, Korea.
2
Department of Orthopedic Surgery, Korea University, Seoul, Korea.
3
Department of Physical Medicine & Rehabilitation, Korea University, Seoul, Korea.
4
Department of Anatomy, Korea University, Seoul, Korea.
5
Biomedical Research Center, Korea Institute of Science and Technology, Seoul, Korea.

Abstract

Platelet-rich plasma containing various growth factors can promote nerve regeneration. An inside-out vein graft can substitute nerve autograft to repair short nerve defects. It is hypothesized that an inside-out vein graft filled with platelet-rich plasma shows better effects in the repair of short sciatic nerve defects. In this study, an inside-out vein autograft filled with platelet-rich plasma was used to bridge a 10 mm-long sciatic nerve defect in rats. The sciatic nerve function of rats with an inside-out vein autograft filled with platelet-rich plasma was better improved than that of rats with a simple inside-out vein autograft. At 6 and 8 weeks, the sciatic nerve function of rats with an inside-out vein autograft filled with platelet-rich plasma was better than that of rats undergoing nerve autografting. Compared with the sciatic nerve repaired with a simple inside-out vein autograft, the number of myelinated axons was higher, axon diameter and myelin sheath were greater in the sciatic nerve repaired with an inside-out vein autograft filled with platelet-rich plasma and they were similar to those in the sciatic nerve repaired with nerve autograft. These findings suggest that an inside-out vein graft filled with platelet-rich plasma can substitute nerve autograft to repair short sciatic nerve defects.

KEYWORDS:

axon diameter; histology; inside-out vein autograft; myelin sheath thickness; myelinated axons; nerve regeneration; neural regeneration; peripheral nerve injury; platelet-rich plasma; sciatic nerve; sciatic nerve index

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