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Nutrition. 2015 Apr;31(4):578-81. doi: 10.1016/j.nut.2014.05.005. Epub 2014 May 29.

Capsaicin-mediated apoptosis of human bladder cancer cells activates dendritic cells via CD91.

Author information

1
Department of Ecological and Biological Sciences, La Tuscia University, Viterbo, Italy.
2
Department of Experimental Medicine, La Sapienza University, Rome, Italy.
3
Department of Molecular Medicine, La Sapienza University, Rome, Italy.
4
Department of Ecological and Biological Sciences, La Tuscia University, Viterbo, Italy. Electronic address: velotti@unitus.it.

Abstract

OBJECTIVES:

Immunostimulation by anticancer cytotoxic drugs is needed for long-term therapeutic success. Activation of dendritic cells (DCs) is crucial to obtain effective and long-lasting anticancer T-cell mediated immunity. The aim of this study was to explore the effect of capsaicin-mediated cell death of bladder cancer cells on the activation of human monocyte-derived CD1a+ immature DCs.

METHODS:

Immature DCs (generated from human peripheral blood-derived CD14+ monocytes cultured with granulocyte-macrophage colony stimulating factor and interleukin-4) were cocultured with capsaicin (CPS)-induced apoptotic bladder cancer cells. DC activation was investigated using immunofluorescence and flow cytometric analysis for key surface molecules. In some experiments, CD91 was silenced in immature DCs.

RESULTS:

We found that capsaicin-mediated cancer cell apoptosis upregulates CD86 and CD83 expression on DCs, indicating the induction of DC activation. Moreover, silencing of CD91 (a common receptor for damage-associated molecular patterns, such as calreticulin and heat-shock protein-90/70) in immature DCs led to the inhibition of DC activation.

CONCLUSIONS:

Our data show that CPS-mediated cancer cell apoptosis activates DCs via CD91, suggesting CPS as an attractive candidate for cancer therapy.

KEYWORDS:

Apoptosis; Bladder cancer; CD91; Capsaicin; Dendritic cells

PMID:
25220876
DOI:
10.1016/j.nut.2014.05.005
[Indexed for MEDLINE]

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