Format

Send to

Choose Destination
Trends Cell Biol. 2014 Nov;24(11):703-11. doi: 10.1016/j.tcb.2014.08.003. Epub 2014 Sep 10.

Architectural proteins: regulators of 3D genome organization in cell fate.

Author information

1
Department of Biology, Emory University, Atlanta, GA 30322, USA.
2
Department of Biology, Emory University, Atlanta, GA 30322, USA. Electronic address: vcorces@emory.edu.

Abstract

The relation between alterations in chromatin structure and changes in gene expression during cell differentiation has served as a paradigm to understand the link between genome organization and function. Yet, the factors involved and the mechanisms by which the 3D organization of the nucleus is established remain poorly understood. The use of Chromosome Conformation-Capture (3C)-based approaches has resulted in a new appreciation of the role of architectural proteins in the establishment of 3D genome organization. Architectural proteins orchestrate higher-order chromatin organization through the establishment of interactions between regulatory elements across multiple spatial scales. The regulation of these proteins, their interaction with DNA, and their co-occurrence in the genome, may be responsible for the plasticity of 3D chromatin architecture that dictates cell and time-specific blueprints of gene expression.

KEYWORDS:

CTCF; chromatin; development; differentiation; transcription

PMID:
25218583
PMCID:
PMC4254322
DOI:
10.1016/j.tcb.2014.08.003
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center