(a) Survival of WT (C57BL/6) and dectin-1 deficient mice, untreated or treated with NEi or AREG, infected intratracheally with 1×107 c.f.u. hgc1Δ yeast-locked C. albicans (n=4). Statistics by Log-rank (Mantel-Cox) test, NS p>0.5, * p<0.05, ** p<0.01. (b) NET release in lungs of WT (C57BL/6) and dectin-1-deficient mice treated with NEi prior to infection or left untreated, infected with 3×106 c.f.u. hgc1Δ yeast-locked C. albicans. NET release 24 h post infection. Lungs sections stained for DNA (DAPI, blue), citrullinated histone H3 (cit-H3, red) and MPO (green) and analyzed by immunofluorescence confocal microscopy. Scale bars = 50 μm. (c) Quantification of fibrin deposition (i, ii) and bleeding (iii, iv) in lungs of WT (C57BL/6) and dectin-1 deficient mice infected with of 3×106 c.f.u. hgc1Δ yeast-locked C. albicans, assessed 36 h post infection with scores from 0 to 3. Average score for each mouse (i, iii) and percent of analyzed images from all mice that fall within each score (ii, iv). Fibrin score: 0 = none, 1 = >1, 2 = <50%, 3 = <50%. Bleeding score: 0 = none, 1 = mild, 2 = moderate, 3 = severe. Statistics by two-way ANOVA, followed by Tukey’s multiple comparison post test: ** p<0.01, *** p<0.001, **** p<0.0001. Data are representative of two independent experiments.