Format

Send to

Choose Destination
Nat Med. 2014 Oct;20(10):1211-6. doi: 10.1038/nm.3640. Epub 2014 Sep 14.

An extracorporeal blood-cleansing device for sepsis therapy.

Author information

1
1] Wyss Institute for Biologically Inspired Engineering, Boston, Massachusetts, USA. [2] Vascular Biology Program, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts, USA. [3] Harvard School of Engineering and Applied Sciences, Cambridge, Massachusetts, USA. [4].
2
1] Wyss Institute for Biologically Inspired Engineering, Boston, Massachusetts, USA. [2].
3
1] Wyss Institute for Biologically Inspired Engineering, Boston, Massachusetts, USA. [2] Vascular Biology Program, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts, USA.
4
1] Wyss Institute for Biologically Inspired Engineering, Boston, Massachusetts, USA. [2] Vascular Biology Program, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts, USA. [3] Harvard-MIT Health Sciences and Technology Graduate Program, Cambridge, Massachusetts, USA.
5
Wyss Institute for Biologically Inspired Engineering, Boston, Massachusetts, USA.
6
Vascular Biology Program, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts, USA.
7
Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
8
1] Wyss Institute for Biologically Inspired Engineering, Boston, Massachusetts, USA. [2] Vascular Biology Program, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts, USA. [3] Harvard School of Engineering and Applied Sciences, Cambridge, Massachusetts, USA.

Abstract

Here we describe a blood-cleansing device for sepsis therapy inspired by the spleen, which can continuously remove pathogens and toxins from blood without first identifying the infectious agent. Blood flowing from an infected individual is mixed with magnetic nanobeads coated with an engineered human opsonin--mannose-binding lectin (MBL)--that captures a broad range of pathogens and toxins without activating complement factors or coagulation. Magnets pull the opsonin-bound pathogens and toxins from the blood; the cleansed blood is then returned back to the individual. The biospleen efficiently removes multiple Gram-negative and Gram-positive bacteria, fungi and endotoxins from whole human blood flowing through a single biospleen unit at up to 1.25 liters per h in vitro. In rats infected with Staphylococcus aureus or Escherichia coli, the biospleen cleared >90% of bacteria from blood, reduced pathogen and immune cell infiltration in multiple organs and decreased inflammatory cytokine levels. In a model of endotoxemic shock, the biospleen increased survival rates after a 5-h treatment.

PMID:
25216635
DOI:
10.1038/nm.3640
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center