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Biotechnol Lett. 2014 Dec;36(12):2545-9. doi: 10.1007/s10529-014-1639-6. Epub 2014 Sep 12.

A comparative proteomic analysis of Bacillus coagulans in response to lactate stress during the production of L-lactic acid.

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College of Pharmacy, Binzhou Medical University, Yantai, 264003, People's Republic of China,


The growth rate and maximum biomass of Bacillus coagulans 2-6 were inhibited by lactate; inhibition by sodium lactate was stronger than by calcium lactate. The differences of protein expressions by B. coagulans 2-6 under the lactate stress were determined using two-dimensional electrophoresis coupled with mass spectrometric identification. Under the non-stress condition, calcium lactate stress and sodium lactate stress, the number of detected protein spots was 1,571 ± 117, 1,281 ± 231 and 904 ± 127, respectively. Four proteins with high expression under lactate stress were identified: lactate dehydrogenase, cysteine synthase A, aldo/keto reductase and ribosomal protein L7/L12. These proteins are thus potential targets for the reconstruction of B. coagulans to promote its resistance to lactate stress.

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