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Methods Mol Biol. 2014;1216:183-96. doi: 10.1007/978-1-4939-1486-9_9.

Identification of protein scaffolds for enzyme design using scaffold selection.

Author information

1
Max Planck Institute for Developmental Biology, Spemannstr. 35, 72076, Tübingen, Germany.

Abstract

The identification of suitable protein structures that can serve as scaffolds for the introduction of catalytic residues is crucial for the design of new enzymes. Here we describe how the automated and rapid scaffold search program ScaffoldSelection can be used to find the best starting points, namely protein structures that are most likely to tolerate the introduction and promote the proper formation of a specific catalytic motif.

PMID:
25213416
DOI:
10.1007/978-1-4939-1486-9_9
[Indexed for MEDLINE]

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