Format

Send to

Choose Destination
Science. 2014 Oct 10;346(6206):248-51. doi: 10.1126/science.1256729. Epub 2014 Sep 11.

Sister kinetochores are mechanically fused during meiosis I in yeast.

Author information

1
Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA.
2
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK.
3
Ludwig Institute for Cancer Research, San Diego Branch, La Jolla, CA 92093, USA.
4
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
5
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK. Institute of Bioanalytics, Department of Biotechnology, Technische Universität Berlin, Berlin, Germany.
6
Ludwig Institute for Cancer Research, San Diego Branch, La Jolla, CA 92093, USA. Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
7
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA. Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.
8
Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK. casbury@u.washington.edu adele.marston@ed.ac.uk.
9
Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA. casbury@u.washington.edu adele.marston@ed.ac.uk.

Abstract

Production of healthy gametes requires a reductional meiosis I division in which replicated sister chromatids comigrate, rather than separate as in mitosis or meiosis II. Fusion of sister kinetochores during meiosis I may underlie sister chromatid comigration in diverse organisms, but direct evidence for such fusion has been lacking. We used laser trapping and quantitative fluorescence microscopy to study native kinetochore particles isolated from yeast. Meiosis I kinetochores formed stronger attachments and carried more microtubule-binding elements than kinetochores isolated from cells in mitosis or meiosis II. The meiosis I-specific monopolin complex was both necessary and sufficient to drive these modifications. Thus, kinetochore fusion directs sister chromatid comigration, a conserved feature of meiosis that is fundamental to Mendelian inheritance.

PMID:
25213378
PMCID:
PMC4226495
DOI:
10.1126/science.1256729
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Loading ...
Support Center