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Br J Cancer. 2014 Nov 11;111(10):2003-13. doi: 10.1038/bjc.2014.485. Epub 2014 Sep 11.

MicroRNA-183 promotes proliferation and invasion in oesophageal squamous cell carcinoma by targeting programmed cell death 4.

Author information

1
1] Department of Gastroenterology, the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province, Nanjing 210029, PR China [2] Department of Gastroenterology, Zhangjiagang First People's Hospital, Affiliated Hospital of Soochow University, Jiangsu Province, Suzhou 215006, PR China.
2
Department of Gastroenterology, the First Affiliated Hospital of Nanjing Medical University, Jiangsu Province, Nanjing 210029, PR China.
3
Department of Gastroenterology, Lianyungang First People's Hospital, Affiliated Hospital of Xuzhou Medical College, Jiangsu Province, Lianyungang 222000, PR China.
4
Department of Gastroenterology, Friendliness Hospital of Yangzhou, Jiangsu Province, Yangzhou 225000, PR China.
5
Department of Gastroenterology, Zhangjiagang First People's Hospital, Affiliated Hospital of Soochow University, Jiangsu Province, Suzhou 215006, PR China.

Abstract

BACKGROUND:

Dysregulated microRNAs (miRNAs) can serve as oncogenes or suppressors and are associated with many cancers, including oesophageal squamous cell carcinoma (ESCC).

METHODS:

An alignment miRNA array was used to identify differentially expressed miRNAs in ESCC tissues. The expression of miR-183 and programmed cell death 4 (PDCD4) in oesophageal tissues from ESCC and early oesophageal carcinoma patients was examined by quantitative reverse transcriptase PCR and western blotting. A luciferase assay was performed to confirm miR-183 target genes. The effects of miR-183 on ESCC cells and the associated mechanisms were established by in vitro experiments.

RESULTS:

We identified 51 upregulated miRNAs and 17 downregulated miRNAs in our array, and miR-183 was one of the most upregulated miRNAs. An inverse correlation between miR-183 and PDCD4 levels was found in ESCC tissues. Upregulated expression of miR-183 was not correlated with tumour stage or lymphatic metastasis in ESCC patients. The luciferase assay confirmed that miR-183 directly interacted with the PDCD4 mRNA 3'-untranslated region in ESCC cells. Overexpression of miR-183 led to decreased PDCD4 protein levels and promoted ESCC cell proliferation and invasion. Inhibition of the PI3K/Akt signalling pathway increased PDCD4 protein levels and decreased miR-183 expression in ESCC cells.

CONCLUSIONS:

MiR-183 promotes ESCC cell proliferation and invasion by directly targeting PDCD4, which suggests that it is involved in the pathogenesis of ESCC.

PMID:
25211657
PMCID:
PMC4229630
DOI:
10.1038/bjc.2014.485
[Indexed for MEDLINE]
Free PMC Article

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