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New Phytol. 2015 Jan;205(2):869-81. doi: 10.1111/nph.13004. Epub 2014 Sep 10.

Insights from the cold transcriptome of Physcomitrella patens: global specialization pattern of conserved transcriptional regulators and identification of orphan genes involved in cold acclimation.

Author information

1
Plant Biotechnology, Faculty of Biology, University of Freiburg, Schänzlestraße 1, D-79104, Freiburg, Germany.

Abstract

The whole-genome transcriptomic cold stress response of the moss Physcomitrella patens was analyzed and correlated with phenotypic and metabolic changes. Based on time-series microarray experiments and quantitative real-time polymerase chain reaction, we characterized the transcriptomic changes related to early stress signaling and the initiation of cold acclimation. Transcription-associated protein (TAP)-encoding genes of P. patens and Arabidopsis thaliana were classified using generalized linear models. Physiological responses were monitored with pulse-amplitude-modulated fluorometry, high-performance liquid chromatography and targeted high-performance mass spectrometry. The transcript levels of 3220 genes were significantly affected by cold. Comparative classification revealed a global specialization of TAP families, a transcript accumulation of transcriptional regulators of the stimulus/stress response and a transcript decline of developmental regulators. Although transcripts of the intermediate to later response are from evolutionarily conserved genes, the early response is dominated by species-specific genes. These orphan genes may encode as yet unknown acclimation processes.

KEYWORDS:

Physcomitrella patens; abiotic stress; abscisic acid (ABA); cold acclimation; microarray; moss; orphan genes; transcriptome

PMID:
25209349
PMCID:
PMC4301180
DOI:
10.1111/nph.13004
[Indexed for MEDLINE]
Free PMC Article

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