Send to

Choose Destination
FASEB J. 2014 Dec;28(12):5311-21. doi: 10.1096/fj.14-258228. Epub 2014 Sep 10.

Lipidomic analyses identify injury-specific phospholipid changes 3 mo after traumatic brain injury.

Author information

Roskamp Institute, Sarasota, Florida, USA; and James A. Haley Veterans Affairs Hospital, Tampa, Florida, USA
Roskamp Institute, Sarasota, Florida, USA; and James A. Haley Veterans Affairs Hospital, Tampa, Florida, USA.
Roskamp Institute, Sarasota, Florida, USA; and.


Phospholipid (PL) abnormalities are observed in the cerebrospinal fluid of patients with traumatic brain injury (TBI), suggesting their role in TBI pathology. Therefore, PL levels were examined in a TBI mouse model that received 1.8 mm deep controlled cortical impact injury or craniectomy only (control). The rotarod and Barnes maze acquisition and probe tests were performed within 2 wk after injury, with another probe test performed 3 mo postinjury. Liquid chromatography/mass spectrometry analyses were performed on lipid extracts from several brain regions and plasma from injured and control mice collected at 3 mo postinjury. Compared to controls, injured mice with sensorimotor and learning deficits had decreased levels of cortical and cerebellar phosphatidylcholine (PC) and phosphatidylethanolamine (PE) levels, while hippocampal PC, sphingomyelin and PE levels were elevated. Ether PE levels were lower in the cortices and plasma of injured animals. Polyunsaturated fatty acid-containing PC and PE species, particularly ratios of docosahexaenoic acid (DHA) to arachidonic acid, were lower in the hippocampi and cortices and plasma of injured mice. Given the importance of DHA in maintaining neuronal function and resolving inflammation and of peroxisomes in synthesis of ether PLs, normalizing these PLs may be a useful strategy for treating the chronic pathology of TBI.


controlled cortical impact; mass spectrometry; phosphatidylcholine; phosphatidylethanolamine; phosphatidylinositol; sphingomyelin

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center