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Methods Mol Biol. 2015;1212:201-7. doi: 10.1007/7651_2014_120.

In vivo stem cell transplantation using reduced cell numbers.

Author information

1
Department of Pharmacology, School of Life Dentistry at Tokyo, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan, ryuryu@tokyo.ndu.ac.jp.

Abstract

Dental pulp stem cell (DPSC) characterization is essential for regeneration of a dentin/pulp like complex in vivo. This is especially important for identifying the potential of DPSCs to function as stem cells. Previously reported DPSC transplantation methods have used with huge numbers of cells, along with hydroxyapatite/tricalcium phosphate (HA/TCP), gelatin and fibrin, and collagen scaffolds. This protocol describe a transplantation protocol that uses fewer cells and a temperature-responsive cell culture dish.

PMID:
25208754
DOI:
10.1007/7651_2014_120
[Indexed for MEDLINE]

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