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Methods Mol Biol. 2016;1304:171-86. doi: 10.1007/7651_2014_113.

Experimental Autoimmune Encephalomyelitis in Marmosets.

Author information

1
Department of Immunobiology, Biomedical Primate Research Centre, 3306, 2280 GH, Rijswijk, The Netherlands, jagessar@bprc.nl.

Abstract

Experimental autoimmune encephalomyelitis (EAE) in the common marmoset, a small-bodied Neotropical primate, is a well-known and validated animal model for multiple sclerosis (MS). This model can be used for exploratory research, i.e., investigating the pathogenic mechanisms involved in MS, and applied research, testing the efficacy of new potential drugs.In this chapter, we will describe a method to induce EAE in the marmoset. In addition, we will explain the most common immunological techniques involved in the marmoset EAE research, namely isolation of mononuclear cells (MNC) from peripheral blood and lymphoid tissue, assaying T cell proliferation by thymidine incorporation, MNC phenotyping by flow cytometry, antibody measurement by ELISA, generation of B cell lines and antigen-specific T cell lines, and assaying cytotoxic T cells.

PMID:
25208751
DOI:
10.1007/7651_2014_113
[Indexed for MEDLINE]

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