Selective binding and reverse transcription inhibition of single-strand poly(A) RNA by metal TMPyP complexes

Inorg Chem. 2014 Oct 6;53(19):10015-7. doi: 10.1021/ic501337c. Epub 2014 Sep 9.

Abstract

Ni-, Cu-, and Zn-TMPyP are capable of binding to single-strand poly(A) RNA with high preference and affinity and inhibiting the reverse transcription of RNA by both M-MuLV and HIV-1 reverse transcriptase. With 10 nM azidothymidine, the IC50 value of M-TMPyP could be lowered to 10(-1) μM order.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites / drug effects
  • Dose-Response Relationship, Drug
  • Metals, Heavy / chemistry
  • Molecular Conformation
  • Moloney murine leukemia virus / enzymology
  • Organometallic Compounds / chemistry
  • Organometallic Compounds / pharmacology*
  • Poly A / antagonists & inhibitors*
  • Poly A / genetics
  • Porphyrins / chemistry
  • RNA, Viral / drug effects*
  • RNA, Viral / genetics
  • RNA-Directed DNA Polymerase / metabolism*
  • Reverse Transcriptase Inhibitors / chemistry
  • Reverse Transcriptase Inhibitors / pharmacology*
  • Structure-Activity Relationship
  • Transcription, Genetic / drug effects*
  • Transcription, Genetic / genetics

Substances

  • Metals, Heavy
  • Organometallic Compounds
  • Porphyrins
  • RNA, Viral
  • Reverse Transcriptase Inhibitors
  • meso-tetrakis(N-methylpyridinium-4-yl)porphyrin
  • Poly A
  • RNA-Directed DNA Polymerase