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Dev Cell. 2014 Sep 8;30(5):569-84. doi: 10.1016/j.devcel.2014.08.001.

Ena/VASP proteins cooperate with the WAVE complex to regulate the actin cytoskeleton.

Author information

1
Cell Motility Laboratory, London Research Institute, Cancer Research UK, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.
2
Institute of Neurobiology, University of Muenster, Badestrasse 9, 48149 Muenster, Germany.
3
Howard Hughes Medical Institute and Department of Biophysics, UT Southwestern Medical Center, Dallas, TX 75390, USA.
4
Institute of Neurobiology, University of Muenster, Badestrasse 9, 48149 Muenster, Germany. Electronic address: sbogdan@uni-muenster.de.
5
Cell Motility Laboratory, London Research Institute, Cancer Research UK, 44 Lincoln's Inn Fields, London WC2A 3LY, UK. Electronic address: michael.way@cancer.org.uk.

Abstract

Ena/VASP proteins and the WAVE regulatory complex (WRC) regulate cell motility by virtue of their ability to independently promote actin polymerization. We demonstrate that Ena/VASP and the WRC control actin polymerization in a cooperative manner through the interaction of the Ena/VASP EVH1 domain with an extended proline rich motif in Abi. This interaction increases cell migration and enables VASP to cooperatively enhance WRC stimulation of Arp2/3 complex-mediated actin assembly in vitro in the presence of Rac. Loss of this interaction in Drosophila macrophages results in defects in lamellipodia formation, cell spreading, and redistribution of Ena to the tips of filopodia-like extensions. Rescue experiments of abi mutants also reveals a physiological requirement for the Abi:Ena interaction in photoreceptor axon targeting and oogenesis. Our data demonstrate that the activities of Ena/VASP and the WRC are intimately linked to ensure optimal control of actin polymerization during cell migration and development.

PMID:
25203209
PMCID:
PMC4165403
DOI:
10.1016/j.devcel.2014.08.001
[Indexed for MEDLINE]
Free PMC Article

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