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Anticancer Res. 2014 Sep;34(9):4747-54.

Development of a novel in vivo cancer model using cell sheet engineering.

Author information

1
Department of Gastroenterological Surgery, Tokyo Women's Medical University, Tokyo, Japan.
2
Department of Gastroenterological Surgery, Tokyo Women's Medical University, Tokyo, Japan Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan aruga.atsushi@twmu.ac.jp.
3
Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.

Abstract

AIM:

Standard in vivo cancer models entail injecting single cancer cells, but this technique is not always successful. We developed a novel cancer cell sheet by using temperature-responsive polymer poly(N-isopropyl acryl amide)-coated plates, which allow controlled attachment and detachment of living cancer cells via simple temperature changes.

MATERIALS AND METHODS:

Four human cancer cell lines were used to make cell sheets. The cancer cell sheets were subcutaneously transplanted into nude mice and compared regarding their tumor-forming ability with the conventional cell suspension technique.

RESULTS:

Human cancer cell sheets were successfully transplanted into nude mice. The cancer cell sheets resulted in stable engraftment and showed a higher tumor volume determined by total flux with the IVIS® imaging system.

CONCLUSION:

Novel cancer cell sheets are useful tools to make in vivo cancer models in mice for the assessment of anticancer therapeutics.

KEYWORDS:

Cancer; cell sheet; mouse model; tissue engineering; transplantation

PMID:
25202053
[Indexed for MEDLINE]

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