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Vox Sang. 2015 Feb;108(2):131-40. doi: 10.1111/vox.12193. Epub 2014 Sep 9.

Metabolomics of AS-5 RBC supernatants following routine storage.

Author information

1
Department of Biochemistry and Molecular Genetics, School of Medicine University of Colorado Denver, Anschutz Medical Campus, Aurora, CO, USA.

Abstract

BACKGROUND AND OBJECTIVES:

The safety and efficacy of stored red blood cells (RBCs) transfusion has been long debated due to retrospective clinical evidence and laboratory results, indicating a potential correlation between increased morbidity and mortality following transfusion of RBC units stored longer than 14 days. We hypothesize that storage in Optisol additive solution-5 leads to a unique metabolomics profile in the supernatant of stored RBCs.

MATERIALS AND METHODS:

Whole blood was drawn from five healthy donors, RBC units were manufactured, and prestorage leucoreduced by filtration. Samples were taken on days 1 and 42, the cells removed, and mass spectrometry-based metabolomics was performed.

RESULTS:

The results confirmed the progressive impairment of RBC energy metabolism by day 42 with indirect markers of a parallel alteration of glutathione and NADPH homeostasis. Moreover, oxidized pro-inflammatory lipids accumulated by the end of storage.

CONCLUSION:

The supernatants from stored RBCs may represent a burden to the transfused recipients from a metabolomics standpoint.

KEYWORDS:

oxidative stress; red blood cell; storage; transfusion medicine

PMID:
25200932
PMCID:
PMC4393710
DOI:
10.1111/vox.12193
[Indexed for MEDLINE]
Free PMC Article

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