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J Biochem Mol Toxicol. 2015 Feb;29(2):70-6. doi: 10.1002/jbt.21669. Epub 2014 Sep 9.

Focal adhesion kinase knockdown in carcinoma-associated fibroblasts inhibits oral squamous cell carcinoma metastasis via downregulating MCP-1/CCL2 expression.

Author information

  • 1Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha, 410008, People's Republic of China. minaj2012@outlook.com.

Abstract

Carcinoma-associated fibroblasts (CAFs) have been demonstrated to play an important role in the occurrence and development of oral squamous cell carcinoma (OSCC). The aim of this study is to investigate the influence of CAFs on OSCC cells and to explore the role of focal adhesion kinase (FAK) in this process. The results showed that oral CAFs expressed a higher level of FAK than normal human gingival fibroblasts (HGFs), and the conditioned medium (CM) of CAFs could induce the invasion and migration of SCC-25, one oral squamous carcinoma cell line. However, knockdown of FAK by small interfering RNA (siRNA) resulted in inhibition of CAF-CM induced cell invasion and migration in SCC-25, probably by reducing the production of monocyte chemoattractant protein-1 (MCP-1/CCL2), one of downstream target chemokines. Therefore, our findings indicated that targeting FAK in CAFs might be a promising strategy for the treatment of OSCC in the future.

KEYWORDS:

-Associated Fibroblasts; Focal Adhesion Kinase; Monocyte Chemoattractant Protein-1; Oral Squamous Cell Carcinoma Carcinoma

PMID:
25199511
DOI:
10.1002/jbt.21669
[PubMed - indexed for MEDLINE]
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