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J Am Chem Soc. 2014 Oct 1;136(39):13709-14. doi: 10.1021/ja505676h. Epub 2014 Sep 23.

Disease detection by ultrasensitive quantification of microdosed synthetic urinary biomarkers.

Author information

1
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Building 76-453, Cambridge, Massachusetts 02139, United States.

Abstract

The delivery of exogenous agents can enable noninvasive disease monitoring, but existing low-dose approaches require complex infrastructure. In this paper, we describe a microdose-scale injectable formulation of nanoparticles that interrogate the activity of thrombin, a key regulator of clotting, and produce urinary reporters of disease state. We establish a customized single molecule detection assay that enables urinary discrimination of thromboembolic disease in mice using doses of the nanoparticulate diagnostic agents that fall under regulatory guidelines for "microdosing."

PMID:
25198059
PMCID:
PMC4183649
DOI:
10.1021/ja505676h
[Indexed for MEDLINE]
Free PMC Article

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