Objectives: Nanos2, an RNA-binding protein, belongs to the Nanos gene-coding family and contains two CCHC zinc-finger motifs. In mouse, it plays a pivotal role in male germ cell development, and self-renewal of spermatogonial stem cells. However, little is known of its expression pattern and functions in dairy goat testis.
Materials and methods: Immunohistochemistry and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) were used to generate the expression profile of Nanos2 in dairy goat testis. Furthermore, its overexpression effects on male germline stem cells (mGSCs) were studied using qRT-PCR, immunofluorescence, dual-luciferase reporter assay and western blotting.
Results: Nanos2 is a conservative gene expressed widely in various tissues, especially in pancreas, and it displays higher expression in adult testes than in other age groups. Overexpression of Nanos2 significantly downregulated meiosis-related genes, including Stra8 and Scp3, which induced inhibition of meiosis. Results from dual-luciferase reporter assay and western blotting indicated that Nanos2 directly downregulated Stra8 in goat GmGSCs.
Conclusions: Taken together, these results suggest that Nanos2 plays an important role in spermatogonia and that its overexpression restrained meiosis in the dairy goat.
© 2014 John Wiley & Sons Ltd.