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Immunol Lett. 2014 Nov;162(1 Pt A):207-11. doi: 10.1016/j.imlet.2014.08.018. Epub 2014 Sep 4.

Association between interleukin 8 -251 A/T and +781 C/T polymorphisms and osteoarthritis risk.

Author information

1
Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
2
Department of Medical Oncology, Cancer Hospital of Wuhan University and Hubei Cancer Hospital, Wuhan 430079, China.
3
Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. Electronic address: shuhyang@yeah.net.

Abstract

PURPOSE:

Interleukin 8 (IL-8), as a member of the CXC chemokine family, has a regulatory role in joint inflammation and cartilage degradation, and contribute to the pathophysiology of osteoarthritis. The aim of the current study was to examine the influence of the IL-8 gene polymorphisms at positions -251 (rs4073) and +781 (rs2227306) on the risk of osteoarthritis.

METHODS:

This hospital-based case-control study comprised 150 patients with osteoarthritis and 150 age- and gender-matched controls. IL-8 251 A/T and +781 C/T polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

RESULTS:

Patients with osteoarthritis had a significantly higher frequency of IL-8 -251 TT genotype [odds ratio (OR)=2.16, 95% confidence interval (CI)=1.09, 4.26; P=0.03], IL-8 -251 T allele (OR=1.41, 95% CI=1.02, 1.94; P=0.04), IL-8 +781 TT genotype (OR=2.79, 95% CI=1.10, 7.08; P=0.03) and IL-8 +781 T allele (OR=1.48, 95% CI=1.02, 2.14; P=0.04) than controls. But the findings are less emphatic by the Bonferroni correction. When stratifying by body mass index, type, articular involvement, and Kellgren-Lawrence grade, no significant differences were found in any groups.

CONCLUSIONS:

For the first time, the current data suggested that the TT genotype and T allele of the IL-8 gene polymorphisms at positions -251 and +781 might confer a high risk of osteoarthritis. In the future, additional well-designed large studies were required for the validation of our results.

KEYWORDS:

Case-control study; Gene polymorphism; Interleukin-8; Osteoarthritis

PMID:
25194757
DOI:
10.1016/j.imlet.2014.08.018
[Indexed for MEDLINE]

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