An improved stool concentration procedure for the detection of Cryptosporidium oocysts in Orang Asli stool samples

Fatmah Md Salleh; Norhayati Moktar; Azlin Mohd Yasin; Hesham M Al-Mekhlafi; Tengku Shahrul Anuar

doi:10.1016/j.mimet.2014.08.019

An improved stool concentration procedure for the detection of Cryptosporidium oocysts in Orang Asli stool samples

J Microbiol Methods. 2014 Nov:106:143-145. doi: 10.1016/j.mimet.2014.08.019. Epub 2014 Sep 2.

Authors

Fatmah Md Salleh¹, Norhayati Moktar¹, Azlin Mohd Yasin¹, Hesham M Al-Mekhlafi², Tengku Shahrul Anuar³

Affiliations

¹ Department of Parasitology and Medical Entomology, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, 56000 Kuala Lumpur, Malaysia.
² Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
³ Department of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA, Puncak Alam Campus, 42300 Selangor, Malaysia. Electronic address: tengku9235@puncakalam.uitm.edu.my.

PMID: 25193442
DOI: 10.1016/j.mimet.2014.08.019

Abstract

To improve the stool concentration procedure, we modified different steps of the standard formalin-ether concentration technique and evaluated these modifications by examining stool samples collected in the field. Seven samples were found positive by the modified formalin-ether concentration technique (M-FECT). Therefore, the M-FECT procedure provides enhanced detection of Cryptosporidium oocysts.

Keywords: Concentration technique; Cryptosporidium oocysts; Formalin–ether.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Child
Child, Preschool
Cryptosporidiosis / diagnosis*
Cryptosporidiosis / microbiology
Cryptosporidium / isolation & purification*
Feces / parasitology*
Female
Humans
Male
Microbiological Techniques / methods*
Middle Aged
Parasitology / methods*
Sensitivity and Specificity
Specimen Handling / methods*