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Biol Sport. 2014 Mar;31(1):73-9. doi: 10.5604/20831862.1093775. Epub 2014 Jan 22.

Effect of exercise training of different intensities on anti-inflammatory reaction in streptozotocin-induced diabetic rats.

Author information

1
Department of Oral Biochemistry, Institute of Oral Bioscience, BK21 program, School of Dentistry ; Department of Sports Science, College of Natural Science ; Department of Physical Education, College of Education, Chonbuk National University, Jeonju, Korea.
2
Department of Oral Biochemistry, Institute of Oral Bioscience, BK21 program, School of Dentistry.
3
Department of Sports Science, College of Natural Science.
4
Department of Physical Education, College of Education, Chonbuk National University, Jeonju, Korea.

Abstract

The study investigated the effect of high- and low-intensity exercise training on inflammatory reaction of blood and skeletal muscle in streptozotocin (STZ)-induced diabetic male Sprague-Dawley rats (243 ± 7 g, 8 weeks). The rats completed treadmill running in either high-intensity exercise (6 weeks of exercise training, acute bouts of exercise) or low-intensity exercise (6 weeks of exercise training). Non-running, sedentary rats served as controls. To induce diabetes mellitus, rats received a peritoneal injection of STZ (50 mg · kg(-1)). Rats were sacrificed immediately after an acute bout of exercise and 6 weeks of exercise training. Inflammatory factors were analyzed by ELISA and by immune blotting from the soleus and extensor digitorum longus muscles. In the serum, inflammatory cytokines (IL-1β, TNF-α, IL-6, IL-4) and reactive oxygen species (ROS) (nitric oxide and malondialdehyde) increased in diabetic rats. However, all exercise training groups displayed reduced inflammatory cytokines and reactive oxygen species. In skeletal muscles, low-intensity exercise training, but not high intensity exercise, reduced the levels of COX-2, iNOS, and MMP-2, which were otherwise markedly elevated in the presence of STZ. Moreover, the levels of GLUT-4 and MyoD were effectively increased by different exercise intensity and exercise duration. Low-intensity exercise training appeared most effective to reduce diabetes-related inflammation. However, high-intensity training also reduced inflammatory factors in tissue-specific muscles. The data implicate regular exercise in protecting against chronic inflammatory diseases, such as diabetes.

KEYWORDS:

GLUT-4; MyoD; ROS; diabetes mellitus; inflammation; intensity exercise training

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