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Mol Nutr Food Res. 2014 Dec;58(12):2379-82. doi: 10.1002/mnfr.201400364. Epub 2014 Oct 2.

Active hexose correlated compound exerts therapeutic effects in lymphocyte driven colitis.

Author information

1
Departments of Pharmacology, CIBERehd, School of Pharmacy, Instituto de Investigación Biosanitaria ibs. GRANADA, University of Granada, Granada, Spain.

Abstract

Active hexose correlated compound (AHCC) is a commercial extract of Basidiomycetes fungi enriched in oligosaccharides that is used as a human nutritional supplement for various purposes in humans. Our aim was to study the anti-inflammatory effect of AHCC in the CD4+ CD62L(+) T cell transfer model of colitis, considered one of the closest to the human disease. Colitis was induced by transfer of CD4(+) CD62L(+) T cells to recombination activating gene 1(-/-) mice. AHCC (75 mg/d) was administered by gavage as a post-treatment. Three groups were established: noncolitic, colitic (CD4(+) CD62L(+) transferred mice treated with vehicle), and AHCC (colitic treated with AHCC). AHCC improved colitis, as evidenced by a 24% lower colonic myeloperoxidase and a 21% lower alkaline phosphatase activity. In addition, a decreased secretion of proinflammatory genes assessed by RT-qPCR was observed, particularly TNF-α and IL-1β. Ex vivo mesenteric lymph node cells obtained from AHCC treated mice exhibited a fully normalized production of IL-6, IL-17, and IL-10 (p < 0.05). Also, AHCC treated mice exhibited decreased STAT4 and IκB-α phosphorylation in splenic CD4(+) cells. Our data provide validation of AHCC colonic anti-inflammatory activity in a chronic, T cell driven model of inflammatory bowel disease.

KEYWORDS:

AHCC; CD4+ CD62L+ T cell; Inflammatory bowel disease; Nutraceutical; Prebiotic

PMID:
25186628
DOI:
10.1002/mnfr.201400364
[Indexed for MEDLINE]

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