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Genes Dev. 2014 Sep 1;28(17):1859-72. doi: 10.1101/gad.248252.114.

A CRISPR view of development.

Author information

1
Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53706, USA;
2
Biochemistry Department, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA;
3
Laboratory of Genetics, Laboratory of Cell and Molecular Biology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
4
Biochemistry Department, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA; wildonger@wisc.edu.

Abstract

The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-associated nuclease 9) system is poised to transform developmental biology by providing a simple, efficient method to precisely manipulate the genome of virtually any developing organism. This RNA-guided nuclease (RGN)-based approach already has been effectively used to induce targeted mutations in multiple genes simultaneously, create conditional alleles, and generate endogenously tagged proteins. Illustrating the adaptability of RGNs, the genomes of >20 different plant and animal species as well as multiple cell lines and primary cells have been successfully modified. Here we review the current and potential uses of RGNs to investigate genome function during development.

KEYWORDS:

CRISPR; Cas9; RNA-guided nuclease; development; genome editing; genome engineering

PMID:
25184674
PMCID:
PMC4197953
DOI:
10.1101/gad.248252.114
[Indexed for MEDLINE]
Free PMC Article

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