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J Clin Virol. 2014 Nov;61(3):406-10. doi: 10.1016/j.jcv.2014.08.010. Epub 2014 Aug 18.

Comparison of three commercial RT-PCR systems for the detection of respiratory viruses.

Author information

1
Department of Pathology and Laboratory Medicine, Albany Medical Center Hospital, Albany, NY, United States.
2
Department of Pathology and Laboratory Medicine, Albany Medical Center Hospital, Albany, NY, United States; Department of Pathology and Laboratory Medicine, Albany Medical College, Albany, NY, United States. Electronic address: stellrk@mail.amc.edu.

Abstract

BACKGROUND:

Due to the insensitivity of rapid tests for respiratory viruses, nucleic acid amplification tests are quickly becoming the standard of care.

OBJECTIVES AND STUDY DESIGN:

The performance of the FilmArray Respiratory Panel (RP) and Verigene RV+ (RV+) were compared in a retrospective analysis of 89 clinical specimens previously determined to be positive for the following viruses by our test of record, Prodesse (Pro): influenza A (29, FluA), influenza B (13, FluB), respiratory syncytial virus (12, RSV), human metapneumovirus (10, hMPV), parainfluenza (14, PIV), and adenovirus (10, AdV). Samples positive for influenza A, B or RSV were tested by both methods, while the remainder were tested by RP only. True positives were defined as positive by two or more assays.

RESULTS:

Limit of detection (LOD) analyses demonstrated Pro had the lowest LOD for all FluA strains tested, PIV1, PIV2 and AdV; RV+ had the lowest LOD for FluB; and RP had the lowest LOD for RSV, PIV3 and hMPV. Of the 55 samples tested by RV+, all 54 true positive samples were positive by RV+. Of the 89 samples tested by RP, 85 of the 88 true positive samples were positive by RP. From these results, the overall sensitivities for influenza A, B and RSV were 100% and 98% for RV+ and RP, respectively. The overall sensitivity of RP for all viruses was 97%.

CONCLUSIONS:

In summary, these systems demonstrated excellent performance. Furthermore, each system has benefits which will ensure they will all have a niche in a clinical laboratory.

KEYWORDS:

Influenza; Multiplex real-time PCR; Respiratory syncytial virus; Respiratory viruses

PMID:
25183359
DOI:
10.1016/j.jcv.2014.08.010
[Indexed for MEDLINE]

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