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PLoS One. 2014 Sep 2;9(9):e104692. doi: 10.1371/journal.pone.0104692. eCollection 2014.

A truncation variant of the cation channel P2RX5 is upregulated during T cell activation.

Author information

1
Institute for Neuroimmunology and Clinical Multiple Sclerosis Research (inims), ZMNH, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Research Department Cell and Gene Therapy, Clinic for Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
2
Institute for Neural Signaltransduction, ZMNH, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
3
Institute for Neuroimmunology and Clinical Multiple Sclerosis Research (inims), ZMNH, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Neuroimmunology and MS Research, Department of Neurology, University Hospital Zurich, Zurich, Switzerland.
4
Institute for Neural Signaltransduction, ZMNH, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Institute for Physiology, University Hospital Homburg, Homburg/Saar, Germany.

Abstract

Members of the P2X family of ligand-gated cation channels (P2RX) are expressed by various cell types including neurons, smooth- and cardiac muscle cells, and leukocytes. The channels mediate signalling in response to extracellular ATP. Seven subunit isoforms (P2RX1-P2RX7) have been identified and these can assemble as homo- and heterotrimeric molecules. In humans, P2RX5 exists as a natural deletion mutant lacking amino acids 328-349 of exon 10, which are part of transmembrane (TM) 2 and pre-TM2 regions in other organisms like rat, chicken and zebrafish. We show that P2RX5 gene expression of human T lymphocytes is upregulated during activation. P2RX5 is recruited to the cell surface. P2RX5-siRNA-transfected CD4+ T cells produced twofold more IL-10 than controls. Surface and intracellular P2RX5 expression was upregulated in activated antigen-specific CD4+ T cell clones. These data indicate a functional role of the human P2RX5 splice variant in T cell activation and immunoregulation.

PMID:
25181038
PMCID:
PMC4152149
DOI:
10.1371/journal.pone.0104692
[Indexed for MEDLINE]
Free PMC Article

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