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Small. 2015 Jan 27;11(4):489-98. doi: 10.1002/smll.201401369. Epub 2014 Sep 2.

Stochastic particle barcoding for single-cell tracking and multiparametric analysis.

Author information

1
Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, 02139, USA.

Abstract

This study presents stochastic particle barcoding (SPB), a method for tracking cell identity across bioanalytical platforms. In this approach, single cells or small collections of cells are co-encapsulated within an enzymatically-degradable hydrogel block along with a random collection of fluorescent beads, whose number, color, and position encode the identity of the cell, enabling samples to be transferred in bulk between single-cell assay platforms without losing the identity of individual cells. The application of SPB is demonstrated for transferring cells from a subnanoliter protein secretion/phenotyping array platform into a microtiter plate, with re-identification accuracies in the plate assay of 96±2%. Encapsulated cells are recovered by digesting the hydrogel, allowing subsequent genotyping and phenotyping of cell lysates. Finally, a model scaling is developed to illustrate how different parameters affect the accuracy of SPB and to motivate scaling of the method to thousands of unique blocks.

KEYWORDS:

barcodes; cells; hydrogels; lab-on-a-chip devices; labeling

PMID:
25180800
PMCID:
PMC4303509
DOI:
10.1002/smll.201401369
[Indexed for MEDLINE]
Free PMC Article

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