Format

Send to

Choose Destination
See comment in PubMed Commons below
PLoS One. 2014 Sep 2;9(9):e106120. doi: 10.1371/journal.pone.0106120. eCollection 2014.

Exercise-induced norepinephrine decreases circulating hematopoietic stem and progenitor cell colony-forming capacity.

Author information

1
Institute of Human Movement Sciences and Sport, Exercise Physiology Lab, ETH Zurich, Zurich, Switzerland; Institute of Biophysics, Medical University of Graz, Graz, Austria.
2
Clinical Institute of Medical and Chemical Laboratory Diagnostics, Research Unit on Lifestyle and Inflammation-associated Risk Biomarkers, Medical University of Graz, Graz, Austria; Institute for Pathophysiology and Immunology, Medical University of Graz, Graz, Austria.
3
Clinical Institute of Medical and Chemical Laboratory Diagnostics, Research Unit on Lifestyle and Inflammation-associated Risk Biomarkers, Medical University of Graz, Graz, Austria; BioTechMed-Graz, Karl-Franzens University & Technical University & Medical University of Graz, Graz, Austria.
4
Department of Ophthalmology, Medical University of Graz, Graz, Austria.
5
Institute for Pathophysiology and Immunology, Medical University of Graz, Graz, Austria.
6
Institute of Sports Science, Karl-Franzens University of Graz, Graz, Austria.
7
Institute of Cell Biology, Histology and Embryology, Medical University of Graz, Graz, Austria.
8
Department of Pulmonology, Medical University of Graz, Graz, Austria.
9
Institute of Biophysics, Medical University of Graz, Graz, Austria.

Abstract

A recent study showed that ergometry increased circulating hematopoietic stem and progenitor cell (CPC) numbers, but reduced hematopoietic colony forming capacity/functionality under normoxia and normobaric hypoxia. Herein we investigated whether an exercise-induced elevated plasma free/bound norepinephrine (NE) concentration could be responsible for directly influencing CPC functionality. Venous blood was taken from ten healthy male subjects (25.3+/-4.4 yrs) before and 4 times after ergometry under normoxia and normobaric hypoxia (FiO2<0.15). The circulating hematopoietic stem and progenitor cell numbers were correlated with free/bound NE, free/bound epinephrine (EPI), cortisol (Co) and interleukin-6 (IL-6). Additionally, the influence of exercise-induced NE and blood lactate (La) on CPC functionality was analyzed in a randomly selected group of subjects (n = 6) in vitro under normoxia by secondary colony-forming unit granulocyte macrophage assays. Concentrations of free NE, EPI, Co and IL-6 were significantly increased post-exercise under normoxia/hypoxia. Ergometry-induced free NE concentrations found in vivo showed a significant impairment of CPC functionality in vitro under normoxia. Thus, ergometry-induced free NE was thought to trigger CPC mobilization 10 minutes post-exercise, but as previously shown impairs CPC proliferative capacity/functionality at the same time. The obtained results suggest that an ergometry-induced free NE concentration has a direct negative effect on CPC functionality. Cortisol may further influence CPC dynamics and functionality.

PMID:
25180783
PMCID:
PMC4152172
DOI:
10.1371/journal.pone.0106120
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Support Center