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EMBO J. 2014 Oct 1;33(19):2188-200. doi: 10.15252/embj.201488677. Epub 2014 Sep 1.

Dictyostelium uses ether-linked inositol phospholipids for intracellular signalling.

Author information

1
Babraham Biosciences Technology Babraham Research Campus, Cambridge, UK.
2
MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK rrk@mrc-lmb.cam.ac.uk len.stephens@bbsrc.ac.uk phillip.hawkins@babraham.ac.uk.
3
MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UK.
4
Signalling Programme Babraham Research Campus, Cambridge, UK.
5
Signalling Programme Babraham Research Campus, Cambridge, UK rrk@mrc-lmb.cam.ac.uk len.stephens@bbsrc.ac.uk phillip.hawkins@babraham.ac.uk.

Abstract

Inositol phospholipids are critical regulators of membrane biology throughout eukaryotes. The general principle by which they perform these roles is conserved across species and involves binding of differentially phosphorylated inositol head groups to specific protein domains. This interaction serves to both recruit and regulate the activity of several different classes of protein which act on membrane surfaces. In mammalian cells, these phosphorylated inositol head groups are predominantly borne by a C38:4 diacylglycerol backbone. We show here that the inositol phospholipids of Dictyostelium are different, being highly enriched in an unusual C34:1e lipid backbone, 1-hexadecyl-2-(11Z-octadecenoyl)-sn-glycero-3-phospho-(1'-myo-inositol), in which the sn-1 position contains an ether-linked C16:0 chain; they are thus plasmanylinositols. These plasmanylinositols respond acutely to stimulation of cells with chemoattractants, and their levels are regulated by PIPKs, PI3Ks and PTEN. In mammals and now in Dictyostelium, the hydrocarbon chains of inositol phospholipids are a highly selected subset of those available to other phospholipids, suggesting that different molecular selectors are at play in these organisms but serve a common, evolutionarily conserved purpose.

KEYWORDS:

Dictyostelium; PI3K; ether lipids; phosphoinositides; plasmanylinositol

Comment in

PMID:
25180230
PMCID:
PMC4282506
DOI:
10.15252/embj.201488677
[Indexed for MEDLINE]
Free PMC Article

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