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Biomed J. 2014 Sep-Oct;37(5):293-7. doi: 10.4103/2319-4170.132887.

Glucose increases interleukin-12 gene expression and production in stimulated peripheral blood mononuclear cells of type 2 diabetes patients.

Author information

1
Division of Pulmonary, Critical Care and Sleep Medicine, Chang Gung Memorial Hospital at Keelung, Chang Gung University College of Medicine, Taoyuan, Taiwan.

Abstract

BACKGROUND:

Lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMCs) of type 2 diabetes patients produce more interleukin (IL)-12 under glucose treatment. The aim of this study was to determine whether increased IL-12 response in hyperglycemic LPS-stimulated PBMCs is due to increased gene expression or osmolarity.

METHODS:

LPS-stimulated PBMCs of 13 type 2 diabetes patients and 8 healthy controls were used for culture in the presence or absence of glucose or mannitol for 24 h. The IL-12 gene expressions of PBMCs and IL-12 protein levels in supernatants were evaluated.

RESULTS:

After 24 h, the stimulated PBMCs of diabetes patients expressed more IL-12 mRNA and produced more IL-12 protein following glucose treatment than those without glucose treatment and with mannitol treatment. Stimulated PBMCs of controls did not express more IL-12 mRNA and produce more IL-12 protein following glucose treatment than those without glucose treatment and with mannitol treatment.

CONCLUSIONS:

Glucose increases the IL-12 production in stimulated PBMCs of diabetes patients through increased IL-12 gene expression.

PMID:
25179705
DOI:
10.4103/2319-4170.132887
[Indexed for MEDLINE]
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