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Curr Opin Struct Biol. 2014 Oct;28:112-21. doi: 10.1016/j.sbi.2014.08.008. Epub 2014 Aug 30.

Quantitative analysis of single-molecule superresolution images.

Author information

1
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
2
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: xiao@jhmi.edu.

Abstract

This review highlights the quantitative capabilities of single-molecule localization-based superresolution imaging methods. In addition to revealing fine structural details, the molecule coordinate lists generated by these methods provide the critical ability to quantify the number, clustering, and colocalization of molecules with 10-50 nm resolution. Here we describe typical workflows and precautions for quantitative analysis of single-molecule superresolution images. These guidelines include potential pitfalls and essential control experiments, allowing critical assessment and interpretation of superresolution images.

PMID:
25179006
PMCID:
PMC4252250
DOI:
10.1016/j.sbi.2014.08.008
[Indexed for MEDLINE]
Free PMC Article

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