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Methods Mol Biol. 2014;1191:175-94. doi: 10.1007/978-1-4939-1170-7_11.

Dynamic analysis of CO₂ labeling and cell respiration using membrane-inlet mass spectrometry.

Author information

1
Genomatica Inc., 4757 Nexus Center Drive, San Diego, CA, 92121, USA, tyang@genomatica.com.

Abstract

Here, we introduce a mass spectrometry-based analytical method and relevant technical details for dynamic cell respiration and CO2 labeling analysis. Such measurements can be utilized as additional information and constraints for model-based (13)C metabolic flux analysis. Dissolved dynamics of oxygen consumption and CO2 mass isotopomer evolution from (13)C-labeled tracer substrates through different cellular processes can be precisely measured on-line using a miniaturized reactor system equipped with a membrane-inlet mass spectrometer. The corresponding specific rates of physiologically relevant gases and CO2 mass isotopomers can be quantified within a short-term range based on the liquid-phase dynamics of dissolved fermentation gases.

PMID:
25178791
DOI:
10.1007/978-1-4939-1170-7_11
[Indexed for MEDLINE]

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