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BMC Vet Res. 2014 Sep 2;10:190. doi: 10.1186/s12917-014-0190-y.

Comparison of the osteogenic potential of mesenchymal stem cells from the bone marrow and adipose tissue of young dogs.

Author information

1
Núcleo de Células Tronco e Terapia Celular Animal, NCT-TCA Departamento de Clínica e Cirurgia, Escola de veterinária da Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. cleuzaufmg@gmail.com.

Abstract

BACKGROUND:

The aim of the present study was to compare the osteogenic potential of mesenchymal stem cells extracted from the bone marrow (BM-MSCs) and adipose tissue (AD-MSCs) of young dogs. The following parameters were assessed: dimethyl thiazolyl diphenyl tetrazolium (MTT) conversion, alkaline phosphatase (ALP) activity, collagen and mineralised matrix synthesis, and the expressions of osterix, bone sialoprotein (BSP), and osteocalcin (OC).

RESULTS:

MTT conversion was greater in BM-MSCs compared to AD-MSCs after 14 and 21 days of differentiation; ALP activity was greater in differentiated AD-MSCs on day 7; collagen synthesis was greater in BM-MSCs on days 14 and 21; the percentage of mineralized area per field was greater in BM-MSCs compared to AD-MSCs; osterix expression was greater in BM-MSCs in days 14 and 21, and BSP and OC expression levels were greater in BM-MSCs at all the investigation time-points.

CONCLUSIONS:

It was concluded that the osteogenic potential was greater in BM-MSCs than AD-MSCs when extracted from young dogs.

PMID:
25178540
PMCID:
PMC4236816
DOI:
10.1186/s12917-014-0190-y
[Indexed for MEDLINE]
Free PMC Article

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