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Tissue Cell. 2014 Dec;46(6):457-61. doi: 10.1016/j.tice.2014.08.002. Epub 2014 Aug 13.

Immunocytochemical study of YB-1 nuclear distribution in different cell types.

Author information

1
Laboratory of Cell Morphology, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Avenue, 194064 St. Petersburg, Russia. Electronic address: ibogol@mail.ru.
2
Institute of Protein Research, Russian Academy of Sciences, 4 Institutskaya Street, 142290 Pushchino, Moscow Region, Russia.
3
Laboratory of Cell Morphology, Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Avenue, 194064 St. Petersburg, Russia.

Abstract

In the present work we studied the distribution of YB-1 in the nuclei of mouse hepatocytes, early embryos and human skin fibroblasts with the use of light and electron microscopy. To reveal YB-1, we applied rat polyclonal antibody against the C-terminal fragment of YB-1 molecule and rabbit polyclonal antibody against full-length YB-1 molecule. YB-1 distribution patterns varied significantly in different cell types. YB-1 was found to be colocalized with RNA polymerase I in mouse hepatocytes and embryos. Besides, YB-1 was revealed in a population of Cajal bodies in 2-cell mouse embryos but not in other cells studied.

KEYWORDS:

Cajal bodies; Cell nucleus; RNA polymerase I; YB-1

PMID:
25175035
DOI:
10.1016/j.tice.2014.08.002
[Indexed for MEDLINE]

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