Immunocytochemical study of YB-1 nuclear distribution in different cell types

Tissue Cell. 2014 Dec;46(6):457-61. doi: 10.1016/j.tice.2014.08.002. Epub 2014 Aug 13.

Abstract

In the present work we studied the distribution of YB-1 in the nuclei of mouse hepatocytes, early embryos and human skin fibroblasts with the use of light and electron microscopy. To reveal YB-1, we applied rat polyclonal antibody against the C-terminal fragment of YB-1 molecule and rabbit polyclonal antibody against full-length YB-1 molecule. YB-1 distribution patterns varied significantly in different cell types. YB-1 was found to be colocalized with RNA polymerase I in mouse hepatocytes and embryos. Besides, YB-1 was revealed in a population of Cajal bodies in 2-cell mouse embryos but not in other cells studied.

Keywords: Cajal bodies; Cell nucleus; RNA polymerase I; YB-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Lineage
  • Cell Nucleus / metabolism
  • Cell Nucleus / ultrastructure
  • Coiled Bodies / ultrastructure
  • Embryo, Mammalian / metabolism
  • Embryo, Mammalian / ultrastructure*
  • Fibroblasts / ultrastructure
  • Hepatocytes / ultrastructure*
  • Humans
  • Immunohistochemistry*
  • Mice
  • Microscopy, Electron
  • Transcription Factors / isolation & purification
  • Transcription Factors / metabolism*

Substances

  • Transcription Factors
  • YB-1 protein, mouse