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Methods Mol Biol. 2014;1213:229-43. doi: 10.1007/978-1-4939-1453-1_19.

Adipogenic fate commitment of muscle-derived progenitor cells: isolation, culture, and differentiation.

Author information

1
Research Group Adipocyte Development, German Institute of Human Nutrition, Arthur-Scheunert-Allee 114-116, Nuthetal, 14558, Germany.

Abstract

Skeletal muscle harbors several types of cells, among which a population of progenitors committed to the adipogenic lineage has only recently been identified. Potential sources of white and brown adipocytes, the latter representing a potential target to treat obesity, are of considerable interest to the field. Fluorescence-activated cell sorting (FACS) provides an elegant strategy for prospective isolation of closely defined cell populations. Here we describe a flow cytometric method to isolate muscle-resident adipogenic progenitor cells with a default potential to undergo white adipogenesis. We further describe an approach to induce commitment to a lineage of brown-like adipocytes upon exposure to bone morphogenetic protein 7 (BMP7).

PMID:
25173387
PMCID:
PMC4511918
DOI:
10.1007/978-1-4939-1453-1_19
[Indexed for MEDLINE]
Free PMC Article

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