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Food Chem. 2015 Feb 1;168:546-53. doi: 10.1016/j.foodchem.2014.07.023. Epub 2014 Jul 12.

Antioxidant activity and protective effects against oxidative damage of human cells induced by X-radiation of phenolic glycosides isolated from pepper fruits Capsicum annuum L.

Author information

1
Research Group of Phytochemistry, Department of Chemistry, University of Life Sciences, ul. Akademicka 15, 20-950 Lublin, Poland. Electronic address: malgorzata.materska@up.lublin.pl.
2
Center for Translational Research and Molecular Biology of Cancer, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology Gliwice Branch, ul. Wybrzeże Armii Krajowej 15, 44-101 Gliwice, Poland.
3
Department of Radiotherapy and Brachytherapy Planning, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology Gliwice Branch, ul. Wybrzeże Armii Krajowej 15, 44-101 Gliwice, Poland.

Abstract

The antioxidant and radioprotective effects of the phenolic glycosides from Capsicum annuum L. were examined. There were: sinapoyl-E-glucoside, quercetin-3-O-rhamnoside-7-O-glucoside, quercetin-3-O-rhamnoside and luteolin-7-O-(2-apiosyl)-glucoside. To the best of our knowledge, this is the first study to assay these compounds for their radioprotective effect on human cell lymphocytes in response to oxidative damage induced by X radiation and their antioxidant abilities. Investigated compounds showed weaker antiradical activities, but their radioprotective potentials were higher than those of their aglycones. Quercetin-3-O-rhamnoside showed the highest radioprotective activity (50% according to control). Furthermore, quercetin and luteolin derivatives, in contrast to free aglycones, were not cytotoxic against human lymphocytes for all concentrations tested. The best correlation between radioprotective and antiradical activities of the investigated compounds was observed in the relationship to O2(-) generated using the NADH/PMS method (R(2)=0.859). Thus, we propose that superoxide radical scavenging activity is a useful method for screening for compounds with promising radioprotective potential.

KEYWORDS:

Antioxidant activity; DNA damages; Glycosides of phenolic compounds; Human lymphocytes; Superoxide radical; X-ray radiation

PMID:
25172746
DOI:
10.1016/j.foodchem.2014.07.023
[Indexed for MEDLINE]

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