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Anal Biochem. 2014 Dec 1;466:49-50. doi: 10.1016/j.ab.2014.08.017. Epub 2014 Aug 27.

A cost-effective approach to microporate mammalian cells with the Neon Transfection System.

Author information

1
Laboratory of Lipid Biochemistry and Protein Interactions, Department of Cellular and Molecular Medicine, KU Leuven (University of Leuven), B-3000 Leuven, Belgium.
2
Laboratory of Lipid Biochemistry and Protein Interactions, Department of Cellular and Molecular Medicine, KU Leuven (University of Leuven), B-3000 Leuven, Belgium. Electronic address: marc.fransen@med.kuleuven.be.

Abstract

Electroporation is one of the most efficient nonviral methods for transferring exogenous DNA into mammalian cells. However, the relatively high costs of electroporation kits and reagents temper the routine use of this fast and easy to perform technique in many laboratories. Several years ago, a new flexible and easy to operate electroporation device was launched under the name Neon Transfection System. This device uses specialized pipette tips containing gold-plated electrodes as electroporation chamber. Here we report a protocol to regenerate these expensive tips as well as some other Neon kit accessories, thereby reducing the cost of electroporation at least 10-fold.

KEYWORDS:

DNase I; Electroporation; Mammalian cells; Microporation; Plasmid DNA; Transfection

PMID:
25172131
DOI:
10.1016/j.ab.2014.08.017
[Indexed for MEDLINE]

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