Format

Send to

Choose Destination
Acta Histochem. 2014 Oct;116(8):1289-300. doi: 10.1016/j.acthis.2014.07.012. Epub 2014 Aug 27.

Expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and peroxiredoxin 6 (Prdx6) proteins in healthy and pathologic placentas of human and rat.

Author information

1
Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
2
Department of Biophysics, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
3
Department of Physiology, Faculty of Medicine, Near East University, Nicosia, Mersin 10, Turkey.
4
Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey. Electronic address: iustunel@akdeniz.edu.tr.

Abstract

A relationship has been shown between preeclampsia (PE) and intrauterine growth restriction (IUGR) and oxidative stress (OS). Since such pregnancies experience OS, we aimed to detect the distribution pattern and expression levels of a transcription factor, Nuclear factor erythroid 2-related factor-2 (Nrf2) that has a role in the regulation of antioxidant enzymes, and peroxiredoxin 6 (Prdx6) an antioxidant enzyme, in human healthy, IUGR, PE and in groups of rat healthy and IUGR placentas using immunohistochemistry and Western blotting. Both Nrf2 and Prdx6 immunoreactivities were weaker in human and rat IUGR group placentas compared to human and rat control group placentas, respectively. Nrf2 and Prdx6 were immunostained in labyrinth trophoblasts, decidua, giant, glycogen and fetal vessel endothelial cells in rat control and IUGR group placentas. Nrf2 and Prdx6 immunoreactivities were seen in the decidua, syncytiotrophoblasts, villous stromal cells, and vascular endothelium in human control, IUGR and PE group placentas. Results of Nrf2 and Prdx6 Western blotting applied for rat and human placentas were compatible with the results of Nrf2 and Prdx6 immunohistochemical observations with regard to rat and human placentas. Down-regulation of Nrf2 and Prdx6 proteins in human and rat IUGR group placentas may have led to the formation of OS which may have impaired proliferation and invasion of cytotrophoblasts.

KEYWORDS:

Human; IUGR; Nrf2; PE; Placenta; Prdx6; Rat

PMID:
25171874
DOI:
10.1016/j.acthis.2014.07.012
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center