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J Allergy Clin Immunol. 2014 Sep;134(3):671-678.e4. doi: 10.1016/j.jaci.2014.06.024.

Enhanced innate type 2 immune response in peripheral blood from patients with asthma.

Author information

1
Division of Allergic Diseases, Department of Internal Medicine, Mayo Clinic, Rochester, Minn; Department of Immunology, Mayo Clinic, Rochester, Minn.
2
Division of Allergic Diseases, Department of Internal Medicine, Mayo Clinic, Rochester, Minn.
3
Division of Allergic Diseases, Department of Internal Medicine, Mayo Clinic, Rochester, Minn; Department of Immunology, Mayo Clinic, Rochester, Minn. Electronic address: kita.hirohito@mayo.edu.

Abstract

BACKGROUND:

In mice, group 2 innate lymphoid cells (ILC2s) likely mediate helminth immunity, inflammation, and tissue repair and remodeling. However, the involvement of ILC2s in human diseases, such as asthma, is not well understood.

OBJECTIVES:

The goals of this study were to investigate whether peripheral blood specimens can be used to monitor innate type 2 immunity in human subjects and to examine whether ILC2s are involved in human asthma.

METHODS:

PBMCs from subjects with allergic asthma (AA), subjects with allergic rhinitis (AR), or healthy control (HC) subjects were cultured in vitro with IL-25 or IL-33. Flow cytometry and cell sorting were used to identify, isolate, and quantitate ILC2s in PBMCs.

RESULTS:

Human PBMCs produced IL-5 and IL-13 when stimulated with IL-33 or IL-25 in the presence of IL-2 without antigens. In addition, IL-7 or thymic stromal lymphopoietin were able to replace IL-2. The cell population with phenotypic ILC2 characteristics, lineage(-)CD127(+)CRTH2(+) cells, responded to IL-33 and produced large quantities of IL-5 and IL-13 but undetectable levels of IL-4. PBMCs from subjects with AA produced significantly larger amounts of IL-5 and IL-13 in response to IL-25 or IL-33 than from subjects with AR or HC. The prevalence of ILC2s in blood was greater in the AA group than in the AR group or the HC group.

CONCLUSIONS:

Innate type 2 immune responses are increased in asthma but not in AR, suggesting potential differences in the immunopathogenesis of these diseases. Peripheral blood is useful for evaluating innate type 2 immunity in humans.

KEYWORDS:

IL-13; IL-25; IL-33; IL-5; ILC2; allergy; asthma; innate immunity

PMID:
25171868
PMCID:
PMC4149890
DOI:
10.1016/j.jaci.2014.06.024
[Indexed for MEDLINE]
Free PMC Article

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