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Asian Pac J Cancer Prev. 2014;15(16):6977-82.

Apoptotic effects of psiRNA-STAT3 on 4T1 breast cancer cells in vitro.

Author information

1
School of Pharmacy, 2Department of Breast Surgery , The Second Clinical Hospital, 3Department of Plastic Surgery, the China- Japan Union Hospital, 4Department of Biology and Medical Engineering, Institute of Regenerative Medicine, Jilin University, Changchun, China E-mail : gbf_cc@sohu.com, zhouqw@jlu.edu.cn.

Abstract

BACKGROUND:

The aim of this study was to investigate the effect of a Lipofectamine2000 (Life2000) Transfection Reagent transfected psiRNA-STAT3 plasmid on 4T1 breast cancer cells.

MATERIALS AND METHODS:

MTT was used to detect the cell proliferation of breast cancer 4T1 cells at different periods (0h, 6h, 8h, 10h); the cell cycle was assessed by flow cytometry; variation of apoptosis and mitochondrial membrane potential was observed under a fluorescence microscope; immunohistochemical staining was used to determine the expression of caspase-3 and cyclin-D1 protein.

RESULTS:

An obvious effect of inhibition to 4T1 cancer cells could be observed at 8h after the psiRNA-STAT3 was transfected. Typical alterations of apoptotic morphological features were visible in the psiRNA-STAT3 treatment group. Mitochondrial membrane potential decreased significantly, the number of cells was increased in G0/G1 phase, and the number of cells was decreased in S phase, and the data were statistically significant (p<0.05), compared with the Scramble and Mock groups. Expression of caspase-3 protein was increased significantly, while that of cyclin D1 was significantly decreased.

CONCLUSIONS:

Life2000 transfected psiRNA-STAT3 plasmid can inhibit 4T1 tumor cell proliferation and promote apoptosis of 4T1 tumor cells, which process depends on the regulation of expression of cyclin D1 and caspase-3 protein.

PMID:
25169471
DOI:
10.7314/apjcp.2014.15.16.6977
[Indexed for MEDLINE]
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