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Int J Parasitol. 2014 Oct 15;44(12):893-9. doi: 10.1016/j.ijpara.2014.07.008. Epub 2014 Aug 23.

Optimal assay design for determining the in vitro sensitivity of ring stage Plasmodium falciparum to artemisinins.

Author information

1
Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, VIC 3010, Australia; Australian Research Council Centre of Excellence for Coherent X-ray Science, The University of Melbourne, Melbourne, VIC 3010, Australia.
2
Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, VIC 3010, Australia; Australian Research Council Centre of Excellence for Coherent X-ray Science, The University of Melbourne, Melbourne, VIC 3010, Australia. Electronic address: ltilley@unimelb.edu.au.

Abstract

Recent reports demonstrate that failure of artemisinin-based antimalarial therapies is associated with an altered response of early blood stage Plasmodium falciparum. This has led to increased interest in the use of pulse assays that mimic clinical drug exposure for analysing artemisinin sensitivity of highly synchronised ring stage parasites. We report a methodology for the reliable execution of drug pulse assays and detail a synchronisation strategy that produces well-defined tightly synchronised ring stage cultures in a convenient time-frame.

KEYWORDS:

Artemisinin; Malaria; Plasmodium falciparum; Pulse drug assay; Synchronisation

PMID:
25161101
DOI:
10.1016/j.ijpara.2014.07.008
[Indexed for MEDLINE]

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