Format

Send to

Choose Destination
See comment in PubMed Commons below
J Proteome Res. 2014 Oct 3;13(10):4377-87. doi: 10.1021/pr500587x. Epub 2014 Sep 3.

A normative study of the synovial fluid proteome from healthy porcine knee joints.

Author information

1
Department of Pathology and Proteomics Center, ‡Department of Orthopaedic Surgery, §Department of Urology, and ∥Howard Hughes Medical Institute, Boston Children's Hospital , Boston, Massachusetts 02115, United States.

Abstract

Synovial fluid in an articulating joint contains proteins derived from the blood plasma and proteins that are produced by cells within the joint tissues, such as synovium, cartilage, ligament, and meniscus. The proteome composition of healthy synovial fluid and the cellular origins of many synovial fluid components are not fully understood. Here, we present a normative proteomics study using porcine synovial fluid. Using our optimized method, we identified 267 proteins with high confidence in healthy synovial fluid. We also evaluated mRNA expression data from tissues that can contribute to the synovial fluid proteome, including synovium, cartilage, blood, and liver, to better estimate the relative contributions from these sources to specific synovial fluid components. We identified 113 proteins in healthy synovial fluid that appear to be primarily derived from plasma transudates, 37 proteins primarily derived from synovium, and 11 proteins primarily derived from cartilage. Finally, we compared the identified synovial fluid proteome to the proteome of human plasma, and we found that the two body fluids share many similarities, underlining the detected plasma derived nature of many synovial fluid components. Knowing the synovial fluid proteome of a healthy joint will help to identify mechanisms that cause joint disease and pathways involved in disease progression.

KEYWORDS:

PTM; Synovial fluid; human; method optimization; origin; plasma; porcine; proteomics; synovium; transcriptomics

PMID:
25160569
PMCID:
PMC4184458
DOI:
10.1021/pr500587x
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society Icon for PubMed Central
    Loading ...
    Support Center